Preparation, Statistical Optimization and In-vitro Characterization of a Dry Powder Inhaler (DPI) Containing Solid Lipid Nanoparticles Encapsulating Amphotericin B: Ion Paired Complexes with Distearoyl Phosphatidylglycerol
Authors
Abstract:
The aim of this study was to prepare dry powder inhalers (DPIs) containing amphotericin B-loaded solid lipid nanoparticles (AMB-SLNs) as an alternative approach for prevention of pulmonary aspergillosis. For solubilizing AMB in small amounts of organic solvents ion paired complexes were firstly formed by establishing electrostatic interaction between AMB and distearoyl phosphatidylglycerol (DSPG). The SLN formulations containing AMB-DSPG complexes were prepared using glycerol monostearate (GMS) as the lipid matrix and soybean lecithin and tween 80 as the surfactants by solvent emulsification-evaporation technique. The nanoparticles were optimized through a fractional factorial design. DPIs were prepared by lyophilization technique using lactose as the inhalational carrier and then after, the formulations were evaluated in terms of aerodynamic particle size distribution using an Andersen cascade impactor. The morphology of the particles was examined using scanning electron microscopy (SEM) and in-vitro drug release profiles were evaluated. Following the statistical results, the particle size, Poly dispersity index (PdI), zeta potential, entrapment efficiency (EE%), and drug loading (DL%) of the optimized SLNs were 187.04 ± 11.97 nm, 0.188 ± 0.028, -30.16 ± 1.6 mV, 89.3 ± 3.47 % and 2.76 ± 0.32 %, respectively. Formulation containing 10% w/v of lactose with the calculated fine particle fraction value as 72.57 ± 4.33% exhibited the appropriate aerodynamic characteristics for pulmonary drug delivery. SEM images revealed de-agglomerated particles. In-vitro release studies showed sustained release of AMB from the carriers and the release kinetics were best fitted to the first order kinetic model.
similar resources
Preparation, statistical optimization and in vitro characterization of solid lipid nanoparticles as a potential vehicle for transdermal delivery of tramadol hydrochloride as a hydrophilic Compound
As encapsulation of hydrophilic drugs in the solid lipid nanoparticles (SLNs) is still a challenging issue, the aim of this study was to prepare SLNs containing tramadol hydrochloride as a hydrophilic compound.The SLNs were prepared using glycerol monostearate (GMS), soy lecithin and tween 80 by double emulsification-solvent evaporation technique. The nanoparticles were optimized through a cent...
full textpreparation, statistical optimization, and in vitro characterization of insulin nanoparticles
full text
Solid Lipid Nanoparticles Containing Nimesulide: Preparation, Characterization and Cytotoxicity Studies
The prospect of improved cancer therapy using Solid Lipid Nanoparticles (SLNs) as drug delivery system is promising. Several obstacles frequently encountered with anticancer compounds, such as poor drug solubility, are overcome by delivering them using SLN. Moreover, the intravenous administration of drugs into SLNs can potentially enhance drug blood circulation time and improve drug performanc...
full textPreparation, characterization, and optimization of primaquine-loaded solid lipid nanoparticles
Primaquine (PQ) is one of the most widely used antimalarial drugs and is the only available drug that combats the relapsing form of malaria. PQ use in higher doses is limited by severe tissue toxicity including hematological- and gastrointestinal-related side effects. Nanoformulation of drugs in an appropriate drug carrier system has been extensively studied and shown to have the potential to i...
full textPreparation, characterization and evaluation of Ginkgo biloba solid lipid nanoparticles
Objective(s): In this work, Ginkgo biloba extract (GBE) loaded solid lipid nanoparticles (SLNs) were synthesized via high pressure homogenization method and their physicochemical properties, as well as cytotoxicity and antibacterial activities were evaluated.Methods: Ginkgo biloba extract SLNs (GBE-SLNs) were prepared using high pressure homogenization method. The morphology and size of S...
full textMy Resources
Journal title
volume 19 issue 3
pages 45- 62
publication date 2020-09-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023